Gukonjesha Kuma kugirango ukureho Solvent hamwe nibitekerezo

Siyanse Yihishe Gukonjesha-Kuma: Kubura Ubushyuhe Buke

A laboratoire ikonjeshaigera ku gukuraho ibisubizo no kwibanda kubintu binyuze mu byiciro bitatu by'ingenzi:

Icyiciro kibanziriza gukonjesha:Ibikoresho birimo ibishishwa bikonjeshwa vuba mubushyuhe buri hagati ya -40 ° C na -80 ° C, bigakora kristu ikomeye.

Kuma Ibanze (Sublimation):Mugihe cyimyuka (mubisanzwe munsi ya 10Pa), kirisiti ya barafu igabanuka mumazi yumuyaga, ikuraho hejuru ya 90% yumuti.

Kuma kabiri (Desorption):Kwiyongera k'ubushyuhe bworoheje (20-40 ° C) byorohereza desorption yuzuye y'amazi aboshye, bigatuma ubuhehere bwa nyuma bwa 1% -5%.

Ubu buryo bukuraho kwangirika kwubushyuhe bukabije kubintu byangiza ubushyuhe, bikarinda imiterere ya molekile ya poroteyine, enzymes, na vitamine. Byongeye kandi, irema imiterere yoroheje yorohereza rehidrasiyo cyangwa gukoresha progaramu itaziguye.

Ibyiza byingenzi bya Laboratoire Yumisha

Ugereranije n'ibikoresho bingana n'inganda, ibyuma byuma bya laboratoire bitanga igenzura risobanutse neza hamwe ninyungu ntoya yo gutunganya:

Kugenzura Ubushyuhe Bwuzuye:"BOTH" ikonjesha moderi yumyeZLGJ-12, kurugero, ikoresha tekinoroji yo gukonjesha yatumijwe mu mahanga, igera ku bushyuhe bwumutego nku -80° C kugirango ubukonje bwihuse.

Ubuyobozi bwa Vacuum bwubwenge:Ibyuma bisobanutse neza bihora bikurikirana urwego rwa vacuum (≤5Pa), hamwe nuburyo bwokwirinda kugirango birinde kugumya.

Gushyushya buhoro buhoro kugirango ushimangire:Bifite ibikoresho byo gushyushya porogaramu zishobora gukoreshwa (nka "BOTH" ikonjesha yumye ya tekinoroji ya PLD igenzura), sisitemu zituma ubushyuhe bwerekeranye nubushyuhe bwibikoresho bitandukanye, bigahindura ibitekerezo.

Kurugero, mubushakashatsi bwa laboratoire yibinyabuzima burimo antibody gukonjesha-gukama, uburyo bwa gakondo bwo guhumeka bwatumye habaho guteranya poroteyine no gukuraho. Ibinyuranye, gukonjesha-gukama ku bushyuhe buke bwarinze hejuru ya 95% yibikorwa bya antibody, ifu yavuyemo ikaba ihagaze neza kubikwa igihe kirekire.

Nka sosiyete izobereye mu bikoresho byo gukonjesha mu gihugu imyaka 17, ibyuma byumye "BYINSHI" byongereye imbaraga zo kuvanaho ibicuruzwa binyuze mu guhanga udushya mu ikoranabuhanga.

1. Sisitemu Yuzuye-Yuzuye Sisitemu yo Gukurikirana

Laboratoire ya "BOTH" ikonjesha ifite ibikoresho byo gukoraho ibara hamwe na module yo kubika amakuru (ishobora kubika inyandiko zigera ku 100.000). Itanga igihe-nyacyo cy'ubushyuhe-vacuum, ifasha abashakashatsi kumenya neza iherezo rya sublimation ya solvent, birinda gukama cyane cyangwa ibibazo bisigaye.

2. Uburyo bwinshi bwo Kurinda Umutekano

Automatic vacuum pump lockout niba ubushyuhe bwumutego buri hejuru ya -50 ° C kugirango wirinde kwangirika.

Ijwi ryumucyo numucyo hamwe nibikorwa byokwirinda byikora mugihe habaye kunanirwa kwa vacuum cyangwa ihindagurika ridasanzwe.

Amashanyarazi ya UPS atabishaka agumana imikorere ya sisitemu yo kugenzura muminota 20 mugihe umuriro wabuze, ukomeza ubushakashatsi.

3. Kwagura imikorere yimikorere

Hamwe na sisitemu yo gutera gazi itabigenewe, gazi ya inert nka azote irashobora kwinjizwa mucyumba cyumye kugirango hirindwe okiside mugihe cyo gukusanya imyanda ya ogisijeni (urugero, Ethanol). Urugereko, rukozwe mu byuma bidafite isuku kandi rufite umutego udafite ingofero, bikuraho ingaruka ziterwa no kwanduza.

Laboratoire ikonjesha ya laboratoire yavuye mubikoresho byoroshye byo kubura umwuma muburyo bukomeye bwo kugenzura ibintu. "BYINSHI"ZLGJUrukurikirane, binyuze mubushishozi bwayo kandi bwa modular, ntabwo itezimbere gusa gukuraho gukuraho ahubwo binashoboza gukurikirana-igihe no gutezimbere uburyo bwo kwibanda. Ku bashakashatsi bashira imbere ubushakashatsi bwuzuye nibisubizo bihamye, ibyo bikoresho birahinduka "umufasha wibikorwa bisanzwe" muri laboratoire.